用户成果
ANTcryo™ 用户已发表 100+ 篇优秀论文。限于篇幅，本页仅列举了少数精选案例和优秀论文。
Featured Case Studies 精选案例
Structures of the Omicron Spike trimer with ACE2 and an anti-Omicron antibody
Xu H et al. · Shanghai Institute of Materia Medica, CAS
ANTcryo™ and UltrAuFoil grids were used to reveal the Omicron mutant strain spike protein structure binding to the therapeutic antibody JMB2002 and receptor ACE2. The final resolutions achieved were 2.69 Å (using ANTcryo™) and 2.77 Å (using UltrAuFoil), demonstrating superior resolution performance.
Omicron 刺突蛋白与 ACE2 及抗 Omicron 抗体复合物的结构
Xu H et al. · 中国科学院上海药物研究所
使用 ANTcryo™ 和 UltrAuFoil 载网揭示 Omicron 突变株刺突蛋白与治疗性抗体 JMB2002 及受体 ACE2 的结合结构。最终分辨率达到 2.69 Å（ANTcryo™）和 2.77 Å（UltrAuFoil），展现出卓越的分辨率性能。
Comparative cryo-EM sample preparation of adrenergic receptors
Stanford University
Comparative analysis using ANTcryo™ and Quantifoil grids for cryo-EM sample preparation of adrenergic receptors. The study achieved resolutions of 2.49 Å with ANTcryo™ and 2.99 Å with Quantifoil, demonstrating superior performance of ANTcryo™ technology.
肾上腺素受体的冷冻电镜样品制备对比分析
Stanford University · 斯坦福大学
使用 ANTcryo™ 与 Quantifoil 载网进行肾上腺素受体的冷冻电镜样品制备对比分析。研究分别达到 2.49 Å（ANTcryo™）和 2.99 Å（Quantifoil）的分辨率，展示了 ANTcryo™ 技术的卓越性能。
Activation and Signaling Mechanism Revealed by Cannabinoid Receptor-Gi Complex Structures
Liu ZJ et al. · ShanghaiTech University
Three-dimensional cryo-EM structures of CB1 and CB2 cannabinoid receptors bound to G protein complexes. Sample preparation used ANTcryo™ grids (formerly CryoMatrix). Final resolutions: CB1 at 3.0 Å, CB2 at 2.9 Å.
大麻素受体–Gi 复合物结构揭示的激活与信号转导机制
Liu ZJ et al. · 上海科技大学
CB1 和 CB2 大麻素受体与 G 蛋白复合物的三维冷冻电镜结构。样品制备使用 ANTcryo™ 载网（原名 CryoMatrix）。最终分辨率：CB1 达 3.0 Å，CB2 达 2.9 Å。
Selected Publications 精选论文
ANTcryo™ 已支持 100+ 篇论文发表。以下为精选选集。
Cell
Structural decoding of reversible covalent linkage of odorants in human olfactory receptor OR6A2
Wang T, Liu ZJ et al. · ShanghaiTech University, iHuman Institute（上海科技大学，iHuman 研究所）
Human olfactory receptor OR6A2 was successfully engineered into a functional protein for the first time, revealing its reversible covalent bond recognition mechanism for aldehyde odorants, and discovering a widely conserved activation switch in Class II olfactory receptors.
DOI: S0092-8674(25)01430-8人类嗅觉受体 OR6A2 中气味分子可逆共价连接的结构解码
Wang T, Liu ZJ et al. · ShanghaiTech University, iHuman Institute（上海科技大学，iHuman 研究所）
人类嗅觉受体 OR6A2 首次被成功改造为功能性蛋白，揭示其对醛类气味分子的可逆共价键识别机制，并发现 II 类嗅觉受体中广泛保守的激活开关。
DOI: S0092-8674(25)01430-8Early fusion intermediate of ACE2-using coronavirus spike acting as an antiviral target
Xing L, Lu L et al. · Fudan University（复旦大学）
First high-resolution structure of a coronavirus S protein early fusion intermediate, revealing the precise conformation of the fusion peptide inserting into the host membrane after S2′ cleavage, and identifying this intermediate as the optimal antiviral target window.
利用 ACE2 的冠状病毒刺突蛋白早期融合中间体作为抗病毒靶点
Xing L, Lu L et al. · Fudan University（复旦大学）
首个冠状病毒 S 蛋白早期融合中间体的高分辨率结构，揭示 S2′ 切割后融合肽插入宿主膜的精确构象，确定该中间体为最佳抗病毒靶点窗口。
Structural Insights into the Diversity and DNA Cleavage Mechanism of Fanzor
Xu P, Saito M, Zhang F et al. · Broad Institute / MIT（博德研究所 / 麻省理工学院）
Structures of 13 Fanzor proteins from 3 different organisms reveal the molecular diversity of eukaryotic gene editing systems; the ωRNA binding interface is highly conserved, while TAM recognition and catalytic sites vary; the RuvC domain 'Lid' loop undergoes conformational changes during guide-DNA pairing to regulate activation.
DOI: S0092-8674(24)00844-4Fanzor 多样性及其 DNA 切割机制的结构洞察
Xu P, Saito M, Zhang F et al. · Broad Institute / MIT（博德研究所 / 麻省理工学院）
来自 3 种不同生物的 13 个 Fanzor 蛋白结构揭示真核基因编辑系统的分子多样性；ωRNA 结合界面高度保守，TAM 识别和催化位点各异；RuvC 结构域 'Lid' 环在 guide-DNA 配对时发生构象变化以调控激活。
DOI: S0092-8674(24)00844-4A potent pan-sarbecovirus neutralizing antibody resilient to epitope diversification
Rosen LE, Starr TN et al. (53 authors) · University of Utah / Vir Biotechnology（犹他大学 / Vir Biotechnology）
Discovery of human monoclonal antibody VIR-7229 with unprecedented cross-reactivity across all sarbecovirus lineages (including non-ACE2 bat sarbecoviruses), while effectively neutralizing all SARS-CoV-2 variants since 2019. Tolerates extreme epitope diversification through high-affinity binding, receptor molecular mimicry, and backbone interactions.
DOI: 10.1016/j.cell.2024.09.026一种耐受表位多样化的高效广谱沙贝冠状病毒中和抗体
Rosen LE, Starr TN et al. (53 authors) · University of Utah / Vir Biotechnology（犹他大学 / Vir Biotechnology）
发现人源单克隆抗体 VIR-7229，对全部 sarbecovirus 进化支（包括非 ACE2 蝙蝠 sarbecovirus）具有前所未有的交叉反应性，同时能有效中和 2019 年以来所有 SARS-CoV-2 变体。通过高亲和力结合、受体分子模拟和骨架相互作用耐受极端表位多样化。
DOI: 10.1016/j.cell.2024.09.026Structure-guided discovery of protein and glycan components in native mastigonemes
Yan N, Yan C, Pan J et al. · Tsinghua University（清华大学）
First native structure of Chlamydomonas flagellar mastigonemes, revealing complex assembly mediated by polysaccharides, and elucidating the molecular basis of ciliary movement and mechanosensation — the only cilia/flagella structural biology work in this collection.
DOI: 10.1016/j.cell.2024.03.005天然鞭毛丝中蛋白与聚糖组分的结构引导发现
Yan N, Yan C, Pan J et al. · Tsinghua University（清华大学）
首个衣藻鞭毛 mastigoneme 天然结构，揭示由多糖介导的复杂组装方式，阐明纤毛运动和机械感知的分子基础——这是该合集中唯一的纤毛/鞭毛结构生物学工作。
DOI: 10.1016/j.cell.2024.03.005Activation and Signaling Mechanism Revealed by Cannabinoid Receptor-Gi Complex Structures
Liu ZJ et al. · ShanghaiTech University（上海科技大学）
Cryo-EM structures of CB1 and CB2 in complex with G proteins. Sample preparation used ANTcryo™ grids. Resolutions: 3.0 Å and 2.9 Å.
DOI: 10.1016/j.cell.2020.01.007大麻素受体–Gi 复合物结构揭示的激活与信号转导机制
Liu ZJ et al. · ShanghaiTech University（上海科技大学）
CB1 和 CB2 与 G 蛋白分子结合的三维冷冻电镜结构。样品制备使用 ANTcryo™ 载网。分辨率：3.0 Å 和 2.9 Å。
DOI: 10.1016/j.cell.2020.01.007Science
Mechanism of DNA targeting by human LINE-1
Jin W, Xu RM et al. · Institute of Biophysics, CAS（中国科学院生物物理研究所）
Reveals the DNA targeting mechanism of human LINE-1 (the only autonomously active retrotransposon). ORF2p functions as a structure-dependent endonuclease, binding upstream dsDNA and recognizing downstream fork/flap structures, suggesting L1 transposition 'hitchhikes' on chromosomal processes with non-canonical DNA structures.
DOI: 10.1126/science.adu3433人类 LINE-1 DNA 靶向机制
Jin W, Xu RM et al. · Institute of Biophysics, CAS（中国科学院生物物理研究所）
揭示人类 LINE-1（唯一自主活跃的逆转座子）的 DNA 靶向机制。ORF2p 作为结构依赖性核酸内切酶发挥作用，结合上游 dsDNA 并识别下游叉状/瓣状结构，提示 L1 转座'搭车'于具有非经典 DNA 结构的染色体过程。
DOI: 10.1126/science.adu3433Structure and function of a huge photosystem I–fucoxanthin chlorophyll supercomplex from a coccolithophore
Shen L, Wang W et al. · Institute of Botany, CAS（中国科学院植物研究所）
Coccolithophore PSI-fucoxanthin supercomplex at 2.79 Å resolution — 38 peripheral FCPI antennas, 819 pigment molecules, 95% quantum efficiency. Demonstrates ANTcryo™'s capability in resolving ultra-large membrane protein complexes.
DOI: 10.1126/science.adv2132颗石藻巨大光系统 I–岩藻黄素叶绿素超复合物的结构与功能
Shen L, Wang W et al. · Institute of Botany, CAS（中国科学院植物研究所）
颗石藻 PSI-岩藻黄素超复合物，分辨率 2.79 Å——38 个外周 FCPI 天线，819 个色素分子，95% 量子效率。展示了 ANTcryo™ 解析超大膜蛋白复合物的能力。
DOI: 10.1126/science.adv2132Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis
Peng R, Chang YW et al. · University of Pennsylvania（宾夕法尼亚大学）
First revelation that influenza virus RNP adopts a right-handed antiparallel double helix structure, with viral RNA wrapped in the minor groove. Visualizes conformational changes of viral polymerase in different functional states; nucleoprotein tail loop identified as a key drug target, with candidate lead compounds provided.
DOI: 10.1126/science.adq7597流感病毒核糖核蛋白复合物组装及持续 RNA 合成的分子基础
Peng R, Chang YW et al. · University of Pennsylvania（宾夕法尼亚大学）
首次揭示流感病毒 RNP 为右手反平行双螺旋结构，病毒 RNA 包裹在小沟中。可视化病毒聚合酶在不同功能状态下的构象变化；核蛋白尾环被确定为关键药物靶点，并提供候选先导化合物。
DOI: 10.1126/science.adq7597Structures of the Omicron Spike trimer with ACE2 and an anti-Omicron antibody
Xu H et al. · Shanghai Institute of Materia Medica, CAS（中国科学院上海药物研究所）
ANTcryo™ vs UltrAuFoil comparison: ANTcryo™ resolution 2.69 Å, UltrAuFoil 2.77 Å.
DOI: 10.1126/science.abn8863Omicron 刺突蛋白与 ACE2 及抗 Omicron 抗体复合物的结构
Xu H et al. · Shanghai Institute of Materia Medica, CAS（中国科学院上海药物研究所）
ANTcryo™ 与 UltrAuFoil 载网对比：ANTcryo™ 分辨率 2.69 Å，UltrAuFoil 2.77 Å。
DOI: 10.1126/science.abn8863Structural basis of glucagon receptor signaling and drug action
Wu B et al. · Shanghai Institute of Materia Medica, CAS（中国科学院上海药物研究所）
Cryo-EM structures of glucagon receptor with Gs and multiple ligand complexes, providing a structural framework for understanding Class B GPCR activation and rational drug design.
DOI: 10.1126/science.aaz5346胰高血糖素受体信号转导与药物作用的结构基础
Wu B et al. · Shanghai Institute of Materia Medica, CAS（中国科学院上海药物研究所）
胰高血糖素受体与 Gs 及多种配体复合物的冷冻电镜结构，为理解 B 类 GPCR 激活和理性药物设计提供结构框架。
DOI: 10.1126/science.aaz5346PNAS
Calcineurin-fused GPCRs enable high-resolution structural studies
Kobilka B et al. · Stanford University（斯坦福大学）
A novel calcineurin fusion strategy for GPCR structural biology, enabling high-resolution cryo-EM studies. This is the first ANTcryo™ publication from an internationally top structural biology laboratory.
DOI: 10.1073/pnas.2414544121钙调磷酸酶融合 GPCR 实现高分辨率结构研究
Kobilka B et al. · Stanford University（斯坦福大学）
一种新颖的钙调磷酸酶融合策略用于 GPCR 结构生物学，实现高分辨率冷冻电镜研究。这是来自国际顶级结构生物学实验室的首篇 ANTcryo™ 发表论文。
DOI: 10.1073/pnas.2414544121Cell Research
Molecular mechanism of the arrestin-biased agonism of neurotensin receptor 1 by an intracellular allosteric modulator
Sun D, Xu HE, Tian C et al. · University of Science and Technology of China（中国科学技术大学）
First high-resolution structure (2.65–2.88 Å) of a GPCR–β-arrestin1–biased allosteric modulator SBI-553 ternary complex. Discovers a new 'loop engagement' coupling mode — NTSR1 ICL3 binds the β-arrestin1 central crest cavity, representing a previously unobserved arrestin-selective GPCR conformation.
DOI: 10.1038/s41422-025-01095-7细胞内变构调节剂介导的神经降压素受体 1 的 arrestin 偏向性激活分子机制
Sun D, Xu HE, Tian C et al. · University of Science and Technology of China（中国科学技术大学）
首个 GPCR–β-arrestin1–偏向性变构调节剂 SBI-553 三元复合物的高分辨率结构（2.65–2.88 Å）。发现一种新的 'loop engagement' 偶联模式——NTSR1 ICL3 结合 β-arrestin1 中央 crest 腔，代表了此前未观察到的 arrestin 选择性 GPCR 构象。
DOI: 10.1038/s41422-025-01095-7Progress in Biophysics and Molecular Biology
Amorphous nickel-titanium alloy film: A novel support for cryo-electron microscopy
Huang F et al. · Institute of Biophysics, CAS（中国科学院生物物理研究所）
Foundational paper introducing amorphous nickel-titanium alloy (ANTA) film as a revolutionary carbon-free cryo-EM support film. Protein adsorption: 0.94 pN vs 16.6 pN (carbon film), 18× reduction; resolution: 2.36 Å vs 2.59 Å (carbon film); conductivity improved by 4 orders of magnitude.
DOI: 10.1016/j.pbiomolbio.2020.07.004非晶镍钛合金膜：一种新型冷冻电镜支撑膜
Huang F et al. · Institute of Biophysics, CAS（中国科学院生物物理研究所）
介绍非晶镍钛合金（ANTA）薄膜作为革命性无碳冷冻电镜支撑膜的基础论文。蛋白吸附：0.94 pN vs 16.6 pN（碳膜），降低 18 倍；分辨率：2.36 Å vs 2.59 Å（碳膜）；电导率提升 4 个数量级。
DOI: 10.1016/j.pbiomolbio.2020.07.004